Tuesday, March 20, 2018

Clinical Trial: The Effect of Royal Jelly Vaginal Gel Compared to IUI Technique on Fertility Rate of Women With Low-Fertility Husbands


Brief Summary:

The present Pocock clinical trial study was conducted In the city of Mashhad in Iran between 2015 and 2016. The study inclusion criteria were men with low fertility of sperm motility < 25% and total motility < 50% (asthenozoospermia), no history of endocrine diseases, no diabetes men, no hormonal problems in their wives, and a healthy salpingography in their wives.

The study subjects were voluntarily assigned to royal gel and IUI groups. In royal gel group, 5 grams of royal gel was used after menstruation and every other night before and after intercourse. IUI group received 75 units of FSH from the second day of the cycle. Then vaginal ultrasound was performed from the sixth day of menstrual cycle to determine the right size of follicle. 10000 units of HCG was administered when follicle diameter reached 16mm, and the study subject was prepared for IUI 32-36 hours later. Each subject alternately swapped groups following fertility failure.

Monday, March 19, 2018

Manuka Honey May Help Prevent, Treat Colon Cancer

Inhibitory effect of Manuka honey on human colon cancer HCT-116 and LoVo cells growth. Part 1: Suppression of proliferation, promotion of apoptosis and arrest of cell cycle 

Food & Function, Accepted Manuscript

Numerous investigations have been made on plant phenolic compounds and cancer prevention in recent decades. Manuka honey (MH) represents a good source of phenolic compounds such as luteolin, kaempferol, quercetin, gallic acid and syringic acid.

The aim of this work was to evaluate the chemopreventive effects of MH on human colon cancer HCT-116 and LoVo cells. Both cells were exposed to different concentration of MH (0-20 mg/mL for HCT-116 cells and 0-60 mg/mL for LoVo cells) for 48 h to measure apoptosis and cell cycle arrest as well as apoptosis and cell cycle regulatory gene and protein expression. MH exhibited profound inhibitory effects on cellular growth by reducing the proliferation ability, inducing apoptosis and arresting cell cycle in a dose-dependent manner.

Interestingly, MH treatment in non-malignant cells did not exert any significant toxicity at similar concentration. The apoptosis event was associated with increasing expression of p53, cleaved-PARP and caspase-3, and with the activation of both intrinsic (caspase-9) and extrinsic (caspase-8) apoptotic pathways. MH induced cell cycle arrest at S phase in HCT-116 cells, simultaneously, in LoVo cells, it arrested at G2/M phase through the modulation of cell cycle regulator genes (cyclin D1, cyclin E, CDK2, CDK4, p21, p27 and Rb). The expression of p-Akt was suppressed while the expression of p-p38MAPK, p-Erk1/2 and endoplasmic stress markers (ATF6 and XBP1) was increased for apoptosis induction.

Overall, these findings indicate that MH could be a promising preventive or curative food therapy for colon cancer.

Sunday, March 18, 2018

Propolis Helps Heal Diabetic Foot Ulcers

Topical propolis improves wound healing in patients with diabetic foot ulcer: a randomized controlled trial

Natural Product Research
Formerly Natural Product Letters
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In this randomized controlled trial, diabetic patients with foot ulcers (Wagner grades 1 and 2) were randomly assigned to conventional therapies for diabetic foot ulcer plus topical propolis ointment (5%; twice daily) or conventional therapies alone.

The process of ulcer healing was observed during 4 weeks and compared between the two groups regarding the size, erythema, exudates, white blood cell (WBC) count and erythrocyte sedimentation rate (ESR). The process of ulcer size reduction during the four-week period of study was significantly different between the groups. However, this difference was not significant between the third and fourth weeks. There was no significant difference between two groups regarding erythema and exudate reduction as well as WBC count and ESR.

Administration of topical propolis ointment in addition to the conventional treatments of diabetic foot ulcer could reduce the size of ulcers with Wagner grades 1 and 2.

Saturday, March 17, 2018

NEW PRODUCT: 2:1 Concentrated Bee Propolis in Veggie Capsules, Produced at Organic and Kosher-Certified Facility, Equals 500 mg of Raw Propolis

NEW PRODUCT: 2:1 Concentrated Bee Propolis in Veggie Capsules, Produced at Organic and Kosher-Certified Facility, Equals 500 mg of Raw Propolis

About the product

• Double-Sealed Bottle to Ensure Freshness and Purity
• 100 Count, Capsule Shell: Vegetable Cellulose
• Bottled in the United States at an NSF, Organic and Kosher Certified-Facility that Adheres to Good Manufacturing Practices (GMP)
• Stringent Quality Control Process Monitors Production from Raw Materials to Finished Product
• Product is Tested and Inspected at Intervals During Manufacture

BUY ApisVita Standardized Bee Propolis

Product description

• Propolis, often called “bee glue,” is a natural resinous compound produced by honey bees from botanical sources and is used to seal openings in the hive and to protect its interior from bacteria and fungi. The word “propolis” is derived from the Greek words “pro” (before) and “polis” (city), meaning “before the city” or “defender of the city.” In this case, the “city” is the hive.

• Propolis contains flavonoids, caffeic acid esters and diterpenic acids, which give it bactericidal, antiviral and antifungal properties.

• Propolisis reputed to have antiseptic, antibacterial, anti-mycotic, astringent, spasmolytic, anti-inflammatory, anaesthetic, antioxidant, anti-tumor, anti-fungal, anti-ulcer, anti-cancer, and immunomodulatory effects.

• Propolis has been used in traditional medicine for millennia. Propolis pellets were even found among the grave goods in Paleolithic and Mesolithic burials of northeastern Italy.

“Throughout their 6,000 year civilization, the Egyptians used propolis medicinally as well as for the mummification of cadavers. The ancient Greeks used propolis to speed up the healing of wounds and Aristotle recommended it for all afflictions of the skin. The Roman legionnaires reportedly carried small amounts of propolis with them into battle, not only to help speed up wound healing but for its analgesic (numbing) properties. The Incas used propolis for infections. During the Boer War, the British used it to keep wounds from becoming infected. Throughout history, propolis has played an important role in veterinary medicine since many of the human uses for propolis are applicable to animals.” (Bee Culture Magazine, 10/21/2016)

Brazilian Red Propolis Shows Antimicrobial, Anti-Inflammatory, Antiparasitic, Antitumor, Antioxidant, Metabolic and Nutraceutical Activities

Biological activities of red propolis: a review

Recent Pat Endocr Metab Immune Drug Discov. 2018 Feb 23

• Background: The red propolis (RdProp) is a resin produced by Apis mellifera bees, which collect the reddish exudate on the surface of its botanic source, the species Dalbergiae castophyllum, popularly known in Brazil as "rabo de bugio". Considered as the 13th type of Brazilian propolis, this resin has been gaining prominence due to its natural composition, rich in bioactive substances not found in other types of propolis.

• Objective: This review aims to address the most important characteristics of PV, its botanical origin, the main constituents, its biological properties and the patents related to this natural product.

• Method: By means of the SciFinder, Google Patents, Patus® and Spacenet, scientific articles and patents involving the term "red propolis" were searched until August 2017

• Results: A number of biological properties, including antimicrobial, anti-inflammatory, antiparasitic, antitumor, antioxidant, metabolic and nutraceutical activities are attributed to RdProp, demonstrating the great potential of its use in the food, pharmaceutical and cosmetics industries.

• Conclusion: The available papers are associated to pharmacological potential of RdProp, but the molecular mechanisms or bioactive compounds responsible for each activity have not yet been fully elucidated.

Friday, March 16, 2018

Royal Jelly May Help Treat Infertility

Protective potential of royal jelly against cadmium-induced infertility in male rats

Andrologia. 2018 Mar 12

This study aimed to investigate the protective potential of Royal jelly (RJ) against cadmium (Cd)-induced testicular dysfunction in rats.

Thirty-five adult male Wistar rats were assigned into five groups. G I; (control) injected intraperitoneally with saline, G II injected intraperitoneally with a single dose of CdCl2 (1 mg/kg BW), G III received RJ (100 mg/kg BW/day) orally, G IV was pre-treated with RJ for 1 week then, treated with CdCl2 , and G V was co-treated with RJ and CdCl2 .

After day 56, serum and tissue samples were collected and analysed. The results showed decreased serum testosterone, luteinising hormone (LH), follicle-stimulating hormone (FSH), superoxide dismutase, glutathione reductase, sperm motility and count while increased malondialdehyde, nitric oxide, tumour necrosis factor-α (TNF-α) and sperm abnormalities, along with a severely damaged seminiferous tubules epithelium with cytoplasmic and nuclear disruptions following Cd toxicity.

Additionally, Cd stimulated testicular mRNA expression of TNF-α while inhibited those of steroidogenic acute regulatory protein, cytochrome P450 cholesterol side chain cleavage enzyme androgen binding protein, FSH-receptor, LH-receptor, androgen receptor, 3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, and cytochrome P450 17A1. These negative alterations of cadmium were greatly reduced by RJ treatment.

This study concluded that RJ protects against Cd-induced testicular toxicity.

Thursday, March 15, 2018

Bee Venom May Help Treat Allergies

Inhibitory effects of bee venom on mast cell-mediated allergic inflammatory responses

Int J Mol Med. 2018 Mar 12

Although bee venom (BV) is a toxin that causes bee stings to be painful, it has been widely used clinically for the treatment of certain immune‑associated diseases. BV has been used traditionally for the treatment of chronic inflammatory diseases.

In this regard, the present study analyzed the effect of BV on the regulation of inflammatory mediator production by mast cells and their allergic inflammatory responses in an animal model. HMC‑1 cells were treated with BV prior to stimulation with phorbol‑12‑myristate 13‑acetate plus calcium ionophore A23187 (PMACI). The production of allergy‑associated pro‑inflammatory mediators was examined, and the underlying mechanisms were investigated. Furthermore, to investigate whether BV exhibits anti‑inflammatory effects associated with anti‑allergic effects in vivo, a compound 48/80‑induced anaphylaxis model was used. BV inhibited histamine release, mRNA expression and production of cytokines in the PMACI‑stimulated HMC‑1 cells. Furthermore, the inhibitory effects of BV on mitogen‑activated protein kinase (MAPK), MAPK kinase, signal transducer and activator of transcription 3 (STAT3) and Akt were demonstrated.

The present study also investigated the ability of BV to inhibit compound 48/80‑induced systemic anaphylaxis in vivo. BV protected the mice against compound 48/80‑induced anaphylactic‑associated mortality. Furthermore, BV suppressed the mRNA expression levels of pro‑inflammatory cytokines, and suppressed the activation of MAPK and STAT3 in this model.

These results provide novel insights into the possible role of BV as a modulator for mast cell‑mediated allergic inflammatory disorders.

Wednesday, March 14, 2018

Bee Venom May Help Treat Chronic Sinusitis

Anti-inflammatory effect of bee venom in an allergic chronic rhinosinusitis mouse model

Mol Med Rep. 2018 Mar 9

Bee venom (BV) has long been used as anti-inflammatory agent in traditional oriental medicine; however, the effect of BV on chronic rhinosinusitis (CRS) is not commonly studied.

The aim of the present study was to determine the anti-inflammatory effect of BV on an allergic CRS mouse model. An allergic CRS mouse model was established following the administration of ovalbumin with Staphylococcus aureus enterotoxin B (SEB) into the nose. A total of 0.5 or 5 ng/ml of BV were intranasally applied 3 times a week for 8 weeks. Histopathological alterations were observed using hematoxylin and eosin, and Periodic acid Schiff staining.

The levels of inflammatory cell infiltration, interleukin (IL)‑4, IL‑10 and interferon (INF)‑γ in nasal lavage fluid (NLF) were measured. Nuclear factor (NF)‑κB and activator protein (AP)‑1 expressions were also determined by immunohistochemical staining. The group treated with BV had significantly decreased inflammatory cell infiltration and PAS‑positive cells. The levels of INF‑γ, and neutrophil and eosinophil counts in NLF were significantly decreased, and the SEB‑induced NF‑κB and AP‑1 expressions in mouse nasal mucosa were significantly suppressed by 0.5 and 5 ng/ml BV.

Thus, BV exerted significant anti‑inflammatory effects in an allergic CRS mouse model and may have potential value for the treatment of CRS.

Tuesday, March 13, 2018

Addition of Propolis Has Anti-Listerial Effect in Milk

Inhibitory activity of propolis against Listeria monocytogenes in milk stored under refrigeration

Food Microbiol. 2018 Aug;73:168-176

Propolis is a natural bee-product with documented antimicrobial properties in vitro. The objective of this study was to develop a protocol for adding propolis into milk and to determine whether the addition of propolis can confer anti-listerial activity during the storage of milk under optimal or improper refrigeration conditions.

Upon dissolving propolis ethanolic extract (PEE) into glycerol, the PEE-glycerol mixture contained no visible insoluble particles and could be dispersed evenly into milk, without leaving any insoluble material. PEE, with or without glycerol, was added into extended shelf-life milk, artificially contaminated with Listeria monocytogenes.

The addition of PEE dissolved into glycerol resulted in a pronounced and dose-dependent anti-listerial effect in milk stored at 4 °C, with the higher concentration tested (4 mg of dry PEE per mL of milk) resulting in complete inhibition of L. monocytogenes growth throughout 30 days of storage. The combination of PEE with glycerol was also effective in significantly reducing the growth rate of the pathogen in milk stored under improper refrigeration (10 °C).

Based on a patented PEE-deodorization protocol, the addition of deodorized PEE into milk resulted in a product with average consumer acceptability. However, the PEE deodorization process resulted in reduction or even complete removal of propolis constituents with known antibacterial activity, with a concomitant significant reduction in its anti-listerial effect.

Nonetheless, the data presented in this manuscript highlight the strong anti-listerial potential of propolis in milk and suggest that, upon further research on its deodorization and standardization, there may be room for the application of propolis as a natural preservative in dairy beverages.

Monday, March 12, 2018

Brazilian Green Propolis May Help Treat Cancer

Anticancer activity of the supercritical extract of Brazilian green propolis and its active component, artepillin C: Bioinformatics and experimental analyses of its mechanisms of action

Int J Oncol. 2018 Mar;52(3):925-932

Propolis, a resinous substance collected by honeybees by mixing their saliva with plant sources, including tree bark and leaves and then mixed with secreted beeswax, possesses a variety of bioactivities.

Whereas caffeic acid phenethyl ester (CAPE) has been recognized as a major bioactive ingredient in New Zealand propolis, Brazilian green propolis, on the other hand, possesses artepillin C (ARC). In this study, we report that, similar to CAPE, ARC docks into and abrogates mortalin-p53 complexes, causing the activation of p53 and the growth arrest of cancer cells.

Cell viability assays using ARC and green propolis-supercritical extract (GPSE) revealed higher cytotoxicity in the latter, supported by nuclear translocation and the activation of p53.

Furthermore, in vivo tumor suppression assays using nude mice, we found that GPSE and its conjugate with γ cyclodextrin (γCD) possessed more potent anticancer activity than purified ARC. GPSE‑γCD may thus be recommended as a natural, effective and economic anticancer amalgam.

Sunday, March 11, 2018

Propolis May Help Treat Celiac Disease

Ex vivo immunomodulatory effect of ethanolic extract of propolis during Celiac Disease: involvement of nitric oxide pathway

Inflammopharmacology. 2018 Mar 7

Celiac Disease (CeD) is a chronic immune-mediated enteropathy, in which dietary gluten induces an inflammatory reaction, predominantly in the duodenum. Propolis is a resinous hive product, collected by honeybees from various plant sources.

Propolis is well-known for its anti-inflammatory, anti-oxidant and immunomodulatory effects, due to its major compounds, polyphenols and flavonoids. The aim of our study was to assess the ex vivo effect of ethanolic extract of propolis (EEP) upon the activity and expression of iNOS, along with IFN-γ and IL-10 production in Algerian Celiac patients. In this context, PBMCs isolated from peripheral blood of Celiac patients and healthy controls were cultured with different concentrations of EEP. NO production was measured using the Griess method, whereas quantitation of IFN-γ and IL-10 levels was performed by ELISA. Inducible nitric oxide synthase (iNOS) expression, NFκB and pSTAT-3 activity were analyzed by immunofluorescence assay.

Our results showed that PBMCs from Celiac patients produced high levels of NO and IFN-γ compared with healthy controls (HC). Interestingly, EEP reduced significantly, NO and IFN-γ levels and significantly increased IL-10 levels at a concentration of 50 µg/mL. Importantly, EEP downmodulated the iNOS expression as well as the activity of NFκB and pSTAT-3 transcription factors.

Altogether, our results highlight the immunomodulatory effect of propolis on NO pathway and on pro-inflammatory cytokines. Therefore, we suggest that propolis may constitute a potential candidate to modulate inflammation during Celiac Disease and has a potential therapeutic value.

Saturday, March 10, 2018

Review of Honey as an Anti-Diabetic Agent

Honey and Diabetes: The Importance of Natural Simple Sugars in Diet for Preventing and Treating Different Type of Diabetes

Oxid Med Cell Longev. 2018 Feb 4;2018:4757893

Diabetes is a metabolic disorder with multifactorial and heterogeneous etiologies. Two types of diabetes are common among humans: type 1 diabetes that occurs when the immune system attacks and destroys insulin and type 2 diabetes, the most common form, that may be caused by several factors, the most important being lifestyle, but also may be determined by different genes.

Honey was used in folk medicine for a long time, but the health benefits were explained in the last decades, when the scientific world was concerned in testing and thus explaining the benefits of honey.

Different studies demonstrate the hypoglycemic effect of honey, but the mechanism of this effect remains unclear.

This review presents the experimental studies completed in the recent years, which support honey as a novel antidiabetic agent that might be of potential significance for the management of diabetes and its complications and also highlights the potential impacts and future perspectives on the use of honey as an antidiabetic agent.

Friday, March 09, 2018

Determination of Aminoglycoside Residues in Honey and Royal Jelly

Optimization and application of parallel solid-phase extraction coupled with ultra-high performance liquid chromatography-tandem mass spectrometry for the determination of 11 aminoglycoside residues in honey and royal jelly

J Chromatogr A. 2018 Feb 19. pii: S0021-9673(18)30211-5

A robust and sensitive method of solid-phase extraction followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established and performed for the simultaneous determination of eleven aminoglycosides (AGs) in royal jelly and honey.

After sample extraction by a phosphate buffer containing trichloroacetic acid (TCA) and ethylenediaminetetracetic acid disodium salt (Na2EDTA), the extraction solution was subjected to a parallel solid-phase extraction for clean-up prior to the LC-MS/MS analysis. The same method was applied to analyze two completely different matrices, honey and royal jelly. Good sensitivity, repeatability, and recovery were obtained by using the mobile phase without an ion-pairing reagent such as heptafluorobutyric acid (HFBA) or sodium heptanesulfonate.

The calibration curves of the honey and royal jelly samples exhibited a good linear response (R2 > 0.99) at six concentrations in the range of 10-1000 μg/mL. The limit of quantification (LOQ) of the AGs ranged from 10 to 25 μg/kg in the honey and from 12.5 to 25 μg/kg in the royal jelly. The recoveries of the AGs for the honey and royal jelly samples were in the range of 79.48% to 108.95% and 74.61% to 113.70% respectively and the relative standard deviations (RSDs) were between 1.23% and 9.59%, and between 1.51% and 9.98%, respectively.

The proposed approach has been allowed in China as a reference method for the simultaneous determination of eleven AGs in honey and royal jelly.

Thursday, March 08, 2018

Propolis Helps Prevent Side Effects of Chemotherapy

Business Standard

Propolis – Propolis or bee glue supports white and red blood cells. It is also a natural anti-infective and an antibiotic. Propolis helps protect the patient from infections and surrounding viruses. Propolis is both available as capsules and in liquid form in pharmacies.

Wednesday, March 07, 2018

Manuka Honey Helps Cure Acne

Can Manuka Honey Really Cure Your Acne?

Manuka honey is the homecoming queen of the beauty world this year. And like the homecoming queen at your high school, it's really good at a lot of things.

But beyond soothing allergies, fighting bacteria, and even helping you sleep better, one of the biggest promises Manuka honey offers is its supposed ability to fight acne. Even celebs like Kourtney Kardashian claim it’s helped zap stubborn zits.

Could slathering this specialty honey from New Zealand bees really clear up your complexion? 

Here's what dermatologists have to say:

Basically, what makes it great for your skin in general makes it really work for acne, says Karen Hammerman, M.D., of Schweiger Dermatology Group.

"Its anti-inflammatory effects can decrease acne inflammation, and as an antibacterial, it leaves few bacteria to infect pores and cause acne," Hammerman says. This should help existing breakouts look less red and swollen, and prevent future painful pimples.

It can also help slough away dead skin cells, she says—keeping them from clogging your pores and causing blackheads and whiteheads. The soothing and hydrating properties of the sweet stuff can heal existing pimples, and it can help speed up recovery from old acne scars, says New York City dermatologist Arash Akhavan, M.D., founder of the Dermatology and Laser Group...